Flow cytometry is a methodology that allows performing simultaneous multi-parametric analysis of single cells in solution. Flow cytometers utilize lasers as light sources to produce both scattered and fluorescent light signals. Cell populations can be characterized and/or purified based on their fluorescent or light scattering characteristics.

Protein expression on cell surface and intracellular molecules can be detected using appropriate fluorescent probes. This technology can be also applied in medium-throughput screening to study the interaction of compounds for a target of interest, for instance by competition binding.

Celtarys Ligands validated for Flow Cytometry assays
  • CELT- 174 hD2 Dopamine receptor fluorescent antagonist (589/616)

    Our potent and selective hD2 Dopamine receptor fluorescent antagonist shows high affinity for hD2 receptor and selectivity over the other receptor subtypes (Ki =1.06 nM for hD2 receptor in radioligand binding assay). It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.

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  • CELT- 426 hD2 Dopamine receptor fluorescent antagonist (560/571)

    Our potent and partially selective hD2 Dopamine receptor fluorescent antagonist shows high affinity for hD2 receptor and partial selectivity over the other receptor subtypes (Ki =89.3 nM for hD2 receptor in radioligand binding assay). It has been validated in Fluorescence Polarization binding assays as a valid alternative to radioligand binding assays.It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.

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  • CELT-240 hD2/D3 Dopamine receptors fluorescent ligand (589/616)

    Our potent hD3/D2 Dopamine receptors fluorescent ligand shows high affinity for hD2 and hD3 receptors (Ki =2.34 nM and 2.14 nM respectively in radioligand binding assays) and selectivity over D4 dopamine receptor. It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.

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