High-content screening (HCS), also known as high-content analysis (HCA), is a largely used methodology in drug discovery to identify compounds interacting with specific targets in whole cells. This assay is especially suited for high throughput screening with multi-well plates.
In a classical competition binding assay, cells are incubated with a fixed concentration of the fluorescent ligand followed by the addition of growing concentrations of the compound of interest (competitor). Fluorescence intensity is measured at each concentration to obtain a sigmoidal curve, suitable for affinity determination.
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CELT-335 hCB1/CB2 Cannabinoids receptors fluorescent ligand (646/662)
View moreOur potent hCB cannabinoids receptors fluorescent ligand shows high affinity for hCB1/CB2 cannabinoids receptor (Ki =44.8 nM and 7.4 nM , respectively in radioligand binding assay). It has been validated in High Content Screening assays and it is suitable also for HTRF binding assays as a valid alternative to radioligand binding assays. It allows to perform cell visualization in fluorescence microscopy and it is potentially suitable for other fluorescence-based assays.
More information and applications
https://www.sciencedirect.com/science/article/pii/S1043661821005545?via%3Dihub
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CELT-331 hCB2 Cannabinoids receptor fluorescent ligand (646/662)
View moreOur potent and selective hCB2 cannabinoids receptor fluorescent ligand shows high affinity for hCB2 cannabinoids receptor and selectivity over hCB1 (Ki =75.9 nM for hCB2 receptor in radioligand binding assay). It is suitable for HTRF binding assays as a valid alternative to radioligand binding assays. It allows to perform cell visualization in fluorescence microscopy, confocal and high content system experiments. It is potentially suitable for other fluorescence-based assays.
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If you want to know more about how to use fluorescent ligands to characterize receptors with pharmacological interest with this and other technologies, click on the button to download the article “Fluorescent ligands: A new method to label your GPCRs”