High-content screening (HCS), also known as high-content analysis (HCA), is a largely used methodology in drug discovery to identify compounds interacting with specific targets in whole cells. This assay is especially suited for high throughput screening with multi-well plates.
In a classical competition binding assay, cells are incubated with a fixed concentration of the fluorescent ligand followed by the addition of growing concentrations of the compound of interest (competitor). Fluorescence intensity is measured at each concentration to obtain a sigmoidal curve, suitable for affinity determination.
Celtarys Ligands validated for High Content Screening assays
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CELT-335 hCB1/CB2 cannabinoids receptors fluorescent ligand (646/662)
For research use only10 µg vial can be used to prepare 72.58 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.
476,00€ View more -
CELT-331 hCB2 cannabinoid receptor fluorescent ligand (646/662)
For research use only10 µg vial can be used to prepare 75.78 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.
476,00€ View more
Do you want more information?
If you want to know more about how to use fluorescent ligands to characterize receptors with pharmacological interest with this and other technologies, click on the button to download the article “Fluorescent ligands: A new method to label your GPCRs”
