High-content screening (HCS), also known as high-content analysis (HCA), is a largely used methodology in drug discovery to identify compounds interacting with specific targets in whole cells. This assay is especially suited for high throughput screening with multi-well plates.
In a classical competition binding assay, cells are incubated with a fixed concentration of the fluorescent ligand followed by the addition of growing concentrations of the compound of interest (competitor). Fluorescence intensity is measured at each concentration to obtain a sigmoidal curve, suitable for affinity determination.
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CELT-335 hCB1/CB2 Cannabinoids receptors fluorescent ligand (646/662)
View moreOur potent hCB cannabinoids receptors fluorescent ligand shows high affinity for hCB1/CB2 cannabinoids receptor (Ki =44.8 nM and 7.4 nM , respectively in radioligand binding assay). It has been validated in High Content Screening assays and it is suitable also for HTRF binding assays as a valid alternative to radioligand binding assays. It allows to perform cell visualization in fluorescence microscopy and it is potentially suitable for other fluorescence-based assays.
More information and applications
https://www.sciencedirect.com/science/article/pii/S1043661821005545?via%3Dihub
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CELT-331 hCB2 Cannabinoids receptor fluorescent ligand (646/662)
View moreOur potent and selective hCB2 cannabinoids receptor fluorescent ligand shows high affinity for hCB2 cannabinoids receptor and selectivity over hCB1 (Ki =75.9 nM for hCB2 receptor in radioligand binding assay). It is suitable for HTRF binding assays as a valid alternative to radioligand binding assays. It allows to perform cell visualization in fluorescence microscopy, confocal and high content system experiments. It is potentially suitable for other fluorescence-based assays.