Our potent and partially selective hD₂ dopamine receptor fluorescent antagonist shows high affinity for hD₂ receptor and partial selectivity over the other receptor subtypes (K_i = 89.3 nM for hD₂ receptor in radioligand binding assay). It has been validated in Fluorescence Polarization binding assays as a valid alternative to radioligand binding assays. It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.