Our potent and selective hD₂ dopamine receptor fluorescent antagonist shows high affinity for hD₂ receptor and selectivity over the other receptor subtypes (K_i = 3.15 nM for hD₂ receptor; and K_i = 294.6 nM and K_i = 220.3 nM for D₃ and D₄ receptors, respectively, in radioligand binding assay). It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.