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Dopamine Receptors D2-like Family
CELT-419 hD3 dopamine receptor fluorescent ligand (560/571) For research use only

Fluorescence and bright-field images of total (left panels) and non-specific binding (central panels) of CELT-419 to live HEK293-D3R cells and to SKOV3 cells without D3 receptors (right panels). HEK293-D3R cells in DMEM medium with added 9% FBS and antibiotic antimycotic solution were incubated with 2 nM CELT-419 in the absence (total binding) or presence (non-specific binding) of 10 μM Spiperone for 2 h in 5% CO2 and at 37 °C. The number of seeded cells per well was 20,000. For negative control, SKOV3 cells without D3 receptors seeded at 30,000 cells per well were used with 2 nM CELT-419. The contrast of fluorescence and bright-field images was enhanced for presentation purposes only, the same lookup table was used for all images of the same imaging mode. The scale bar corresponds to 10 μm.

Saturation binding of CELT-419 binding to D3 receptors on live HEK293-D3R cells. The HEK293-D3R cells (20,000 cells per well) were incubated with CELT-419 (twofold serial dilutions up to 4 nM) for 4.3 h. Non-specific binding (orange triangles) was measured in the presence of 10 μM Spiperone. The calculated Kd value is given as mean ± SEM of three independent experiments.

Our potent and partially selective hD₃ dopamine receptor fluorescent ligand shows high affinity for hD₃ receptor and partial selectivity over the other receptor subtypes (K_i = 65.6 nM for hD₃ receptor in radioligand binding assay). It has been validated in Fluorescence Polarization binding assays as a valid alternative to radioligand binding assays. It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.

More information and applications

Article: Fluorescence based HTS-compatible ligand binding assays for dopamine D3 receptors in baculovirus preparations and live cells.

CELT-419 hD3 dopamine receptor fluorescent ligand (560/571)
_{For research use only}

Name: CELT-419 hD3 dopamine receptor fluorescent ligand (560/571) For research use only - Celtarys
Price: 476.00 EUR
Availability: InStock

10 µg vial can be used to prepare 75.18 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

476,00€

In stock

Category: Dopamine Receptors D2-like Family

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More information and contact in info@celtarys.com

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More information and applications

CELT-419 hD3 dopamine receptor fluorescent ligand (560/571) For research use only

10 µg vial can be used to prepare 75.18 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.Please note that the required concentration may vary depending on the specific assay design.

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CELT-419 hD3 dopamine receptor fluorescent ligand (560/571)
_{For research use only}

10 µg vial can be used to prepare 75.18 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

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10 µg vial can be used to prepare 78.30 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

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10 µg vial can be used to prepare 63.38 µL of a 100 µM stock solution, providing enough volume for 6 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

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10 µg vial can be used to prepare 73.24 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

CELT-240 hD2/D3 dopamine receptors fluorescent ligand (589/616)
_{For research use only}

10 µg vial can be used to prepare 77.93 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.