E3 ligase kits

Our E3 ligase kits provide everything you need for binary complex assays involving E3 ligases, right out of the box.

CRBN and VHL E3 ligases both belong to the Cullin-RING ligase family, meaning they are composed of multiple proteins. In both cases, CRBN and VHL act as substrate receptors that recognize the target and bind to their respective adaptor proteins, DDB1 and ELOB/C.

Our kits include DDB1–CRBN and ELOB/C–VHL constructs, which simulate physiological binding conditions.

The included fluorescent tracers have very high affinity, in the low nanomolar range, and enable fast implementation through fluorescence polarization, a technique that requires fewer assay steps.

Accelerate your workflow and improve consistency across experiments with our ready-to-use kits.

CRBN Binding Assay Kit (FP-384 well plate) For PROTAC and molecular glue drug discovery

Complete fluorescence polarization (FP)-based assay kit for evaluating ligand binding to the CUL4A/RBX1/DDB1/CRBN complex. The kit includes a high-affinity fluorescent tracer (CELT-077) together with the CUL4A/RBX1/DDB1/CRBN protein complex, providing a ready-to-use system for quantitative binding studies.
1.200,00€
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CRBN Binding Assay Kit (FP-96 well plate) For PROTAC and molecular glue drug discovery

Complete fluorescence polarization (FP)-based assay kit for evaluating ligand binding to the CUL4A/RBX1/DDB1/CRBN complex. The kit includes a high-affinity fluorescent tracer (CELT-077) together with the CUL4A/RBX1/DDB1/CRBN protein complex, providing a ready-to-use system for quantitative binding studies.
SPECIAL 30 % DISCOUNT FOR PRODUCT LAUNCH!
Original price was: 900,00€.Current price is: 630,00€.
SPECIAL 30 % DISCOUNT FOR PRODUCT LAUNCH!

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VHL Binding Assay Kit (FP-384 well plate) For PROTAC and molecular glue drug discovery

Complete fluorescence polarization (FP)-based assay kit to evaluate ligand binding to the VHL E3 ligase complex. The kit includes a high-affinity fluorescent tracer (CELT-504) together with the VBC protein complex, providing a ready-to-use system for quantitative binding studies.
1.200,00€
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VHL Binding Assay Kit (FP-96 well plate) For PROTAC and molecular glue drug discovery

Complete fluorescence polarization (FP)-based assay kit to evaluate ligand binding to the VHL E3 ligase complex. The kit includes a high-affinity fluorescent tracer (CELT-504) together with the VBC protein complex, providing a ready-to-use system for quantitative binding studies.
SPECIAL 30 % DISCOUNT FOR PRODUCT LAUNCH!
Original price was: 900,00€.Current price is: 630,00€.
SPECIAL 30 % DISCOUNT FOR PRODUCT LAUNCH!

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VHL Cooperativity Assay Kit (FP-384 well plate)

Fluorescence polarization (FP)-based assay designed to evaluate the apparent cooperativity of VHL-recruiting PROTACs by comparing their binding affinities in the absence and presence of a protein of interest (POI).
1.200,00€
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