Our potent hD₂/D₃ dopamine receptors fluorescent ligand shows a high affinity for hD₂ and hD₃ receptors (K_i = 5.22 nM and 4.77 nM respectively in radioligand binding assays) and selectivity over D₄ dopamine receptor. It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.