Our potent pan-human 5-HT₂ serotonin receptor fluorescent ligand shows affinity for 5-HT₂A, 5-HT₂B and 5-HT₂C serotonin receptors (K_i = 4.9 nM, 53.0 nM and 47.8 nM, respectively, in radioligand binding assays). It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.