Shop
Our VHL binding assay kit is a fluorescence polarization (FP)-based assay that enables quantitative evaluation of ligand binding to the VHL E3 ubiquitin ligase complex (VBC). The VHL E3 ligase is part of the Cullin-RING ligase family, meaning it functions as a multi-protein complex. VHL acts as the substrate receptor, recognizing the target (physiologically HIF-1α) and binding to its adaptor proteins, ELOB/C, which connect it to the rest of the complex.
This kit uses CELT-504 as a high-affinity fluorescent tracer (low nanomolar range), allowing the determination of IC₅₀ and Ki values through competitive binding experiments in a robust binary format.
The assay has been validated using reference compounds such as VH298 and MZ1, providing affinity values consistent with reported data.
The homogeneous FP format allows direct monitoring of binding events without washing steps, making it suitable for compound screening, profiling and PROTAC development workflows.
For more information on fluorescence polarization techniques, please refer to our blog article.
VHL Binding Assay Kit (FP-96 well plate) For PROTAC and molecular glue drug discovery
Complete fluorescence polarization (FP)-based assay kit to evaluate ligand binding to the VHL E3 ligase complex. The kit includes a high-affinity fluorescent tracer (CELT-504) together with the VBC protein complex, providing a ready-to-use system for quantitative binding studies.
For research use only.Original price was: 900,00€.630,00€Current price is: 630,00€.
SPECIAL 30 % DISCOUNT FOR PRODUCT LAUNCH!
Related products
-
CRBN Binding Assay Kit (FP-96 well plate) For PROTAC and molecular glue drug discovery
Complete fluorescence polarization (FP)-based assay kit for evaluating ligand binding to the CUL4A/RBX1/DDB1/CRBN complex. The kit includes a high-affinity fluorescent tracer (CELT-077) together with the CUL4A/RBX1/DDB1/CRBN protein complex, providing a ready-to-use system for quantitative binding studies.
SPECIAL 30 % DISCOUNT FOR PRODUCT LAUNCH! Original price was: 900,00€.630,00€Current price is: 630,00€.View moreSPECIAL 30 % DISCOUNT FOR PRODUCT LAUNCH!
-
VHL Binding Assay Kit (FP-384 well plate) For PROTAC and molecular glue drug discovery
Complete fluorescence polarization (FP)-based assay kit to evaluate ligand binding to the VHL E3 ligase complex. The kit includes a high-affinity fluorescent tracer (CELT-504) together with the VBC protein complex, providing a ready-to-use system for quantitative binding studies.
1.200,00€ View more -
CRBN Binding Assay Kit (FP-384 well plate) For PROTAC and molecular glue drug discovery
Complete fluorescence polarization (FP)-based assay kit for evaluating ligand binding to the CUL4A/RBX1/DDB1/CRBN complex. The kit includes a high-affinity fluorescent tracer (CELT-077) together with the CUL4A/RBX1/DDB1/CRBN protein complex, providing a ready-to-use system for quantitative binding studies.
1.200,00€ View more -
VHL Cooperativity Assay Kit (FP-384 well plate)
Fluorescence polarization (FP)-based assay designed to evaluate the apparent cooperativity of VHL-recruiting PROTACs by comparing their binding affinities in the absence and presence of a protein of interest (POI).
1.200,00€ View more
Esta entidad fue beneficiaria de las ayudas para la ejecución de acciones de promoción exterior de las empresas gallegas. El objetivo principal de estas ayudas es incentivar la realización de acciones de promoción exterior generadoras de ventajas competitivas. El resultado que se pretende alcanzar es el impulso de las pymes y sus productos y servicios, aumentando el número de empresas de base exportadora.
PERTE SALUD Proyecto Nº: IDI-2025-0751
Título: TERAPIAS AVANZADAS EN INMUNOONCOLOGÍA: UNA ESTRATEGIA INNOVADORA
BASADA EN LA DEGRADACIÓN DIRIGIDA DE GPCR EN EL EJE ADENOSINÉRGICOS
EIC Pathfinder; Project name: Unisens; Universal GPCR Activity Sensor for Next Generation Drug Discovery
Esta entidad fue beneficiaria de las ayudas para la ejecución de acciones de promoción exterior de las empresas gallegas. El objetivo principal de estas ayudas es incentivar la realización de acciones de promoción exterior generadoras de ventajas competitivas. El resultado que se pretende conseguir es el impulso de las pymes y sus productos y servicios, aumentando el número de empresas de base exportadora.
Celtarys Research is beneficiary of a WomenTechEU grant supporting deep-tech start-ups led by women
Principia 2021 Program
Celtarys staff has been funded by the Xunta de Galicia within the framework of the PRINCIPIA 2021 Program of the Galician Innovation Agency (GAIN)
InnovaPEME Program
Celtarys Innovation Plan has been funded by the InnovaPEME 2022 program of the Galician Innovation Agency (GAIN)
Bonos de Innovación de Celtarys Research (026)
Para promover o desenvolvemento tecnolóxico, a innovación e unha investigación de calidade. Esta operación está financiada pola Xunta de Galicia, a través de axudas concedidas pola Axencia Galega de Innovación, dentro do programa de axudas a empresa Bonos de innovación 2022.
Celtarys Research is part of the public-private consortium developing the project PREDICTEAM
New prognostic quantitative biomarker assays for predicting patient response to immune checkpoint inhibitor (ICI) treatment
NEOTEC · CDTI — Ministerio de Ciencia e Innovación
The Project ‘New chemical conjugation technology for therapeutic targets’ has been founded by CDTI under its program NEOTEC. The NEOTEC program objective is supporting the establishment and consolidation of technology-based enterprises. A technology-based company (EBT) is a company whose activity focuses on the exploitation of products or services that require the use of technologies and knowledge developed since the research activity. The EBT base their business strategy or activity in the intensive domain of scientific and technical knowledge. Budget awarded: 416.177€ | Execution dates: 1/1/2023 – 31/03/2024