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CELT-483 sigma hσ1/σ2 receptors fluorescent ligand (646/662) For research use only

Representative confocal microscopy images showing the co-localization of Compound 19 (CELT-483) with σ2 receptor expression in MCF7 cells. Cells were preincubated with 10 μM selective σ1 receptor agonist (+)-pentazocine for 2 h to mask residual σ1 receptor expression. This was followed by incubation for 1 h with the indicated fluorescent ligand at 5 μM. Then cells were fixed with paraformaldehyde, stained for σ2 receptor expression (TMEM97 ab) as well as nuclei (by DAPI), and analyzed by confocal microscopy. The ligand is colored red (scale bar of 20 μm).

Representative spinning disk images showing the localization of ligand Compound 19 (CELT-483) with the mitochondria as well as reduction of the Cy-5 signal in the presence of the nonfluorescent σ2 ligand DTG in living MCF7 cells. Cells were preincubated with 10 μM Compound 31 to mask residual σ1 receptor expression with or without 20 μM DTG for 75 min. This was followed by administration of 0.05 μM Compound 19 (CELT-483) for 30 min (red stain in the Cy-5 channel). In addition, mitochondria were stained with 250 nM MitoTracker-Bodipy FL (green stain in the GFP channel) and the nuclei were counterstained with Hoechst 33342 (blue stain in the DAPI channel). The depicted scale bar indicates 20 μm.

Our potent sigma hσ₁/σ₂ receptor fluorescent ligand is available in a 10 µg vial, allowing you to prepare 103 mL of 100 nM working solution to test σ₁/σ₂ receptors. Our CELT-483 product has been specifically designed to meet the needs of researchers in a variety of fields, enabling the study of σ₁ and σ₂ receptors with accuracy and precision. It has been validated in flow cytometry (K_d = 13.59 nM for σ₂) and confocal microcopy in MCF7 cell lines (see publication for more details) to study both σ₁ and σ₂receptors, using the appropriate masking agent.

CELT-483 sigma hσ₁/σ₂ receptors fluorescent ligand (646/662)
_{For research use only}

Name: CELT-483 sigma hσ 1 /σ 2 receptors fluorescent ligand (646/662) For research use only - Celtarys
Price: 476.00 EUR
Availability: InStock

10 µg vial can be used to prepare 95.03 µL of a 100 µM stock solution, providing enough volume for 9 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

476,00€

In stock

Category: Intracellular Receptors ligands

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Data Sheet CELT 483

More information and applications

More information and contact in info@celtarys.com

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BOX OFFER – CELT-483 with masking agents
_{For research use only}

10 µg vial can be used to prepare 95.03 µL of a 100 µM stock solution, providing enough volume for 9 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.
539,00€
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PERTE SALUD Proyecto Nº: IDI-2025-0751

Título: TERAPIAS AVANZADAS EN INMUNOONCOLOGÍA: UNA ESTRATEGIA INNOVADORA

BASADA EN LA DEGRADACIÓN DIRIGIDA DE GPCR EN EL EJE ADENOSINÉRGICOS

EIC Pathfinder; Project name: Unisens; Universal GPCR Activity Sensor for Next Generation Drug Discovery

Esta entidad fue beneficiaria de las ayudas para la ejecución de acciones de promoción exterior de las empresas gallegas. El objetivo principal de estas ayudas es incentivar la realización de acciones de promoción exterior generadoras de ventajas competitivas. El resultado que se pretende conseguir es el impulso de las pymes y sus productos y servicios, aumentando el número de empresas de base exportadora.

Celtarys Research is beneficiary of a WomenTechEU grant supporting deep-tech start-ups led by women

Principia 2021 Program
Celtarys staff has been funded by the Xunta de Galicia within the framework of the PRINCIPIA 2021 Program of the Galician Innovation Agency (GAIN)

InnovaPEME Program
Celtarys Innovation Plan has been funded by the InnovaPEME 2022 program of the Galician Innovation Agency (GAIN)

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Para promover o desenvolvemento tecnolóxico, a innovación e unha investigación de calidade. Esta operación está financiada pola Xunta de Galicia, a través de axudas concedidas pola Axencia Galega de Innovación, dentro do programa de axudas a empresa Bonos de innovación 2022.

Celtarys Research is part of the public-private consortium developing the project PREDICTEAM

New prognostic quantitative biomarker assays for predicting patient response to immune checkpoint inhibitor (ICI) treatment

NEOTEC · CDTI — Ministerio de Ciencia e Innovación

The Project ‘New chemical conjugation technology for therapeutic targets’ has been founded by CDTI under its program NEOTEC. The NEOTEC program objective is supporting the establishment and consolidation of technology-based enterprises. A technology-based company (EBT) is a company whose activity focuses on the exploitation of products or services that require the use of technologies and knowledge developed since the research activity. The EBT base their business strategy or activity in the intensive domain of scientific and technical knowledge. Budget awarded: 416.177€ | Execution dates: 1/1/2023 – 31/03/2024

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CELT-483 sigma hσ1/σ2 receptors fluorescent ligand (646/662) For research use only

10 µg vial can be used to prepare 95.03 µL of a 100 µM stock solution, providing enough volume for 9 assays at a working concentration of 100 nM.Please note that the required concentration may vary depending on the specific assay design.

You may also like…

BOX OFFER – CELT-483 with masking agents For research use only

10 µg vial can be used to prepare 95.03 µL of a 100 µM stock solution, providing enough volume for 9 assays at a working concentration of 100 nM.Please note that the required concentration may vary depending on the specific assay design.

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10 µg vial can be used to prepare 95.03 µL of a 100 µM stock solution, providing enough volume for 9 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

BOX OFFER – CELT-483 with masking agents
_{For research use only}

10 µg vial can be used to prepare 95.03 µL of a 100 µM stock solution, providing enough volume for 9 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

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CELT-503 fluorescent GTPγS – Red Emission Spectrum (589/616)
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50 µg vial can be used to prepare 38.97 µL of a 1 mM stock solution, providing enough volume for 38 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

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_{For research use only}

10 µg vial can be used to prepare 77.93 µL of a 100 µM stock solution, providing enough volume for 7 assays at a working concentration of 100 nM.
Please note that the required concentration may vary depending on the specific assay design.

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_{For research use only}

3 nmol vial that allows to prepare 30 µL of 100 µM stock solution to test GLP1R/GIPR. One vial is sufficient for injecting one mouse.
Please note that the required concentration may vary depending on the specific assay design.